|Can a few grams of Sustamine™, i.e. L‑Alanylglutamine, prevent protein breakdown after an intense workout? How significant are reduced AMPK and NF-kB protein levels? And lastly: Do we even care about 20 min?|
Due to its increased stability and correspondingly higher absorption rates for intact glutamine, AlaGln dipeptide facilitate greater increases in plasma glutamine concentration compared with glutamine or wheat protein, which has the highest glutamine content of all common protein formulas (Harris. 2012; Rogero. 2006).
Cruzat et al. report in their 2010 study that AlaGln administration can attenuate muscle damage, but their results are based exclusively on the observation of lower inflammation biomarkers following prolonged endurance exercise (Cruzat et al. 2010). A more significant outcome parameter was measured recently by Wang et al. (2015). In their study, the scientists investigated
"the effects of acute Sustamine™ (SUS) supplementation, a dipeptide composed of alanine and glutamine, on the signaling pathways controlling MPS and MPB post resistance exercise. Comparisons were made relative to whey protein (WP) supplementation" (Wang. 2015).In that it is a bit disappointing that the subjects" of the study were eighty-nine male Sprague–Dawley rats who had been familiarized with the standardized exercise protocol, before they had to do latter climbing 10 times with a weight equal to 75 % of their body mass attached at the base of the tail.
|Figure 1: Levels of the alleged markers of protein breakdown, NF-kB, AMPK and FOXO3A, 20 min (A) and 40 min (B) after the workout (Wang. 2015).|Figure 2: Unfortunately, Wang et al. forget that NF-kB is only "bad" for your muscle if it's chronically elevated (Kramer. 2007)
- SUS supplements had no effect on mTOR signaling, but WP supplementation yielded a greater phosphorylation of mTOR p70S6k, and rpS6 compared with PLA at 20 min post exercise.
- By 40 min post exercise, phosphorylation of mTOR and rpS6 in PLA had risen to levels not different than WP.
- Kramer, Henning F., and Laurie J. Goodyear. "Exercise, MAPK, and NF-κB signaling in skeletal muscle." Journal of Applied Physiology 103.1 (2007): 388-395.
- Li, Hong, Shweta Malhotra, and Ashok Kumar. "Nuclear factor-kappa B signaling in skeletal muscle atrophy." Journal of molecular medicine 86.10 (2008): 1113-1126.
- Sandri, Marco, et al. "Foxo transcription factors induce the atrophy-related ubiquitin ligase atrogin-1 and cause skeletal muscle atrophy." Cell 117.3 (2004): 399-412.
- Wang, Wanyi, et al. "l-Alanylglutamine inhibits signaling proteins that activate protein degradation, but does not affect proteins that activate protein synthesis after an acute resistance exercise." Amino acids (2015): 1-10.